Enumeration of Bacteriophages by Double Agar Overlay Plaque Assay

Kropinski, Andrew M.; Mazzocco, Amanda; Waddell, Thomas E.; Lingohr, Erika J.; Johnson, Roger P.

doi:10.1007/978-1-60327-164-6_7

articleMethods in molecular biologyJan 1, 2009Closed access

Enumeration of Bacteriophages by Double Agar Overlay Plaque Assay

AMAndrew M. Kropinski AMAmanda MazzoccoTEThomas E. WaddellEJErika J. Lingohr RPRoger P. Johnson

Public Health Agency of Canada

PubMed

Indexed incrossrefpubmed

Abstract

The determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics, and molecular biology. In this chapter the classical overlay protocol is described.

Citation impact

1,073

total citations

FWCI: 11.96
Percentile: 100%
References: 23

Citations per year

Authors

AM
Andrew M. KropinskiCorresponding
Public Health Agency of Canada
AM
Amanda Mazzocco
TE
Thomas E. Waddell
EJ
Erika J. Lingohr
Public Health Agency of Canada
RP
Roger P. Johnson
Public Health Agency of Canada

Topics & keywords

Topics

Bacteriophages and microbial interactions100%
RNA and protein synthesis mechanisms99%
Monoclonal and Polyclonal Antibodies Research99%

Keywords

Enumeration
Agar
Virus quantification
Microbiology
Overlay
Biology
Computer science
Bacteria

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