Watching a Protein as it Functions with 150-ps Time-Resolved X-ray Crystallography
National Institutes of Health · University of Wisconsin–Madison · +5 more institutions
Abstract
We report picosecond time-resolved x-ray diffraction from the myoglobin (Mb) mutant in which Leu 29 is replaced by Phe (L29Fmutant). The frame-by-frame structural evolution, resolved to 1.8 angstroms, allows one to literally “watch” the protein as it executes its function. Time-resolved mid-infrared spectroscopy of flash-photolyzed L29F MbCO revealed a short-lived CO intermediate whose 140-ps lifetime is shorter than that found in wild-type protein by a factor of 1000. The electron density maps of the protein unveil transient conformational changes far more dramatic than the structural differences between the carboxy and deoxy states and depict the correlated side-chain motion responsible for rapidly sweeping…
Citation impact
- FWCI
- 18.78
- Percentile
- 100%
- References
- 21
Authors
9- FSFriedrich SchotteCorresponding
National Institutes of Health, University of Wisconsin–Madison, Harvard University, National Institute of Diabetes and Digestive and Kidney Diseases, Pusan National University, Rice University
- MLManho Lim
National Institutes of Health, University of Wisconsin–Madison, Harvard University, National Institute of Diabetes and Digestive and Kidney Diseases, Pusan National University, Rice University
- TATimothy A. Jackson
National Institutes of Health, University of Wisconsin–Madison, Harvard University, National Institute of Diabetes and Digestive and Kidney Diseases, Pusan National University, Rice University
- АВА. В. Смирнов
National Institutes of Health, University of Wisconsin–Madison, Harvard University, National Institute of Diabetes and Digestive and Kidney Diseases, Pusan National University, Rice University
- JSJayashree Soman
National Institutes of Health, University of Wisconsin–Madison, Harvard University, National Institute of Diabetes and Digestive and Kidney Diseases, Pusan National University, Rice University
Topics & keywords
- Myoglobin
- Picosecond
- Crystallography
- Chemistry
- X-ray crystallography
- Protein structure
- Angstrom
- Diffraction