Exosomes from human saliva as a source of microRNA biomarkers
National Institute of Dental and Craniofacial Research · Asociación Colombiana de Diabetes · +1 more institution
Abstract
The aim of this study was to examine the presence of microRNAs (miRNAs) within exosomes isolated from human saliva and to optimize and test methods for successful downstream applications.
Exosomes isolated from fresh and frozen glandular and whole human saliva were used as a source of miRNAs. The presence of miRNAs was validated with TaqMan quantitative PCR and miRNA microarrays.
Citation impact
- FWCI
- 18.39
- Percentile
- 100%
- References
- 23
Authors
7- AMA MichaelCorresponding
National Institute of Dental and Craniofacial Research, Asociación Colombiana de Diabetes
- SBSD Bajracharya
National Institute of Dental and Craniofacial Research, Asociación Colombiana de Diabetes
- PYPST Yuen
National Institute of Diabetes and Digestive and Kidney Diseases
- HZHuandi Zhou
National Institute of Diabetes and Digestive and Kidney Diseases
- RSRA Star
National Institute of Diabetes and Digestive and Kidney Diseases
Topics & keywords
- Microvesicles
- Saliva
- microRNA
- TaqMan
- Microarray
- Exosome
- DNA microarray
- Biology