A survey of human brain transcriptome diversity at the single cell level

The human brain is a tissue of vast complexity in terms of the cell types it comprises. Conventional approaches to classifying cell types in the human brain at single cell resolution have been limited to exploring relatively few markers and therefore have provided a limited molecular characterization of any given cell type. We used single cell RNA sequencing on 466 cells to capture the cellular complexity of the adult and fetal human brain at a whole transcriptome level. Healthy adult temporal lobe tissue was obtained during surgical procedures where otherwise normal tissue was removed to gain access to deeper hippocampal pathology in patients with medical refractory seizures. We were able to classify…

• CI
  California Institute for Regenerative Medicine

  Award: GC1R-06673
• SS
  Svenska Sällskapet för Medicinsk Forskning
• NI
  National Institutes of Health

  Awards: F30MH106261, T32GM007365