Biodiversity soup: metabarcoding of arthropods for rapid biodiversity assessment and biomonitoring

Summary 1. Traditional biodiversity assessment is costly in time, money and taxonomic expertise. Moreover, data are frequently collected in ways (e.g. visual bird lists) that are unsuitable for auditing by neutral parties, which is necessary for dispute resolution. 2. We present protocols for the extraction of ecological, taxonomic and phylogenetic information from bulk samples of arthropods. The protocols combine mass trapping of arthropods, mass‐PCR amplification of the COI barcode gene, pyrosequencing and bioinformatic analysis, which together we call ‘metabarcoding’. 3. We construct seven communities of arthropods (mostly insects) and show that it is possible to recover a substantial proportion of the…