An inexpensive, automation‐friendly protocol for recovering high‐quality DNA

Ivanova, Natalya; deWaard, Jeremy R; Hebert, Paul D. N.

doi:10.1111/j.1471-8286.2006.01428.x

articleMolecular Ecology NotesJul 7, 2006Closed access

An inexpensive, automation‐friendly protocol for recovering high‐quality DNA

NINatalya Ivanova JRJeremy R deWaard PDPaul D. N. Hebert

University of Guelph

Indexed incrossref

Abstract

Abstract Although commercial kits are available for automated DNA extraction, ‘artisanal’ protocols are not. In this study, we present a silica‐based method that is sensitive, inexpensive and compliant with automation. The effectiveness of this protocol has now been tested on more than 5000 animal specimens with highly positive results.

Citation impact

1,473

total citations

FWCI: 9.81
Percentile: 100%
References: 8

Citations per year

Authors

NI
Natalya IvanovaCorresponding
University of Guelph
JR
Jeremy R deWaard
University of Guelph
PD
Paul D. N. Hebert
University of Guelph

Topics & keywords

Topics

Identification and Quantification in Food100%
Environmental DNA in Biodiversity Studies100%
Genomics and Phylogenetic Studies99%

Keywords

Automation
Protocol (science)
DNA extraction
Computer science
Embedded system
Biology
Engineering
Medicine

No related works found for this paper.

Funding

GA
Gordon and Betty Moore Foundation
GC
Genome Canada
OG
Ontario Genomics Institute
CR
Canada Research Chairs
NS
Natural Sciences and Engineering Research Council of Canada