Iron and Pseudomonas aeruginosa biofilm formation

Iron serves as a signal in Pseudomonas aeruginosa biofilm development. We examined the influence of mutations in known and putative iron acquisition-signaling genes on biofilm morphology. In iron-sufficient medium, mutants that cannot obtain iron through the high-affinity pyoverdine iron acquisition system form thin biofilms similar to those formed by the parent under low iron conditions. If an iron source for a different iron acquisition system is provided to a pyoverdine mutant, normal biofilm development occurs. This enabled us to identify iron uptake gene clusters that likely serve in transport of ferric citrate and ferrioxamine. We suggest that the functional iron signal for P. aeruginosa biofilm…

• WM
  W. M. Keck Foundation
• UO
  University of Washington
• NI
  National Institute of General Medical Sciences

  Award: GM59026
• NI
  National Institute of Allergy and Infectious Diseases