Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction

Corman, Victor M.; Eckerle, Isabella; Bleicker, Tobias; Zaki, Ali M.; Landt, Olfert; Eschbach-Bludau, Monika; Boheemen, Sander van; Gopal, Robin; Ballhause, Matthias; Bestebroer, Theo M.; Muth, Doreen; Müller, Marcel A.; Drexler, Jan Felix; Zambon, Maria; Osterhaus, Albert D. M. E.; Fouchier, Ron A. M.; Drosten, Christian

doi:10.2807/ese.17.39.20285-en

articleEurosurveillanceSep 27, 2012DIAMOND OA

Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction

VMVictor M. Corman IEIsabella Eckerle TBTobias Bleicker AMAli M. Zaki OLOlfert Landt

University of Bonn · German Center for Infection Research · +3 more institutions

PubMed

Indexed incrossrefdoajpubmed

Abstract

We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5–6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.

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581

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Topics & keywords

Topics

Keywords

Reverse transcription polymerase chain reaction
Reverse transcriptase
Polymerase chain reaction
Coronavirus
Real-time polymerase chain reaction
Virology
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Coronavirus disease 2019 (COVID-19)

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