Neuroprotectin D1: A docosahexaenoic acid-derived docosatriene protects human retinal pigment epithelial cells from oxidative stress
Brigham and Women's Hospital · Harvard University · +1 more institution
Abstract
Docosahexaenoic acid (DHA) is a lipid peroxidation target in oxidative injury to retinal pigment epithelium (RPE) and retina. Photoreceptor and synaptic membranes share the highest content of DHA of all cell membranes. This fatty acid is required for RPE functional integrity; however, it is not known whether specific mediators generated from DHA contribute to its biological significance. We used human ARPE-19 cells and demonstrated the synthesis of 10,17S-docosatriene [neuroprotectin D1 (NPD1)]. This synthesis was enhanced by the calcium ionophore A-23187, by IL-1beta, or by supplying DHA. Under these conditions, there is a time-dependent release of endogenous free DHA followed by NPD1 formation, suggesting…
Citation impact
- FWCI
- 35.10
- Percentile
- 100%
- References
- 50
Authors
4- PKPranab K. Mukherjee
Brigham and Women's Hospital, Harvard University, Louisiana State University Health Sciences Center New Orleans
- VLVictor L. Marcheselli
Brigham and Women's Hospital, Harvard University, Louisiana State University Health Sciences Center New Orleans
- CNCharles N. Serhan
Brigham and Women's Hospital, Harvard University, Louisiana State University Health Sciences Center New Orleans
- NGNicolás G. BazánCorresponding
Brigham and Women's Hospital, Harvard University, Louisiana State University Health Sciences Center New Orleans
Topics & keywords
- Docosahexaenoic acid
- Oxidative stress
- Cell biology
- Apoptosis
- Lipid signaling
- Retinal
- Biology
- Lipid peroxidation