Light-induced cell damage in live-cell super-resolution microscopy
University of Würzburg · Stiftung Juliusspital
Abstract
Super-resolution microscopy can unravel previously hidden details of cellular structures but requires high irradiation intensities to use the limited photon budget efficiently. Such high photon densities are likely to induce cellular damage in live-cell experiments. We applied single-molecule localization microscopy conditions and tested the influence of irradiation intensity, illumination-mode, wavelength, light-dose, temperature and fluorescence labeling on the survival probability of different cell lines 20-24 hours after irradiation. In addition, we measured the microtubule growth speed after irradiation. The photo-sensitivity is dramatically increased at lower irradiation wavelength. We observed fixation,…
Citation impact
- FWCI
- 55.17
- Percentile
- 100%
- References
- 49
Authors
5- SWSina WäldchenCorresponding
University of Würzburg, Stiftung Juliusspital
- JLJulian Lehmann
University of Würzburg, Stiftung Juliusspital
- TKTeresa Klein
University of Würzburg, Stiftung Juliusspital
- SVSebastian van de Linde
University of Würzburg, Stiftung Juliusspital
- MSMarkus Sauer
University of Würzburg, Stiftung Juliusspital
Topics & keywords
- Irradiation
- Microscopy
- Fluorescence microscope
- Biophysics
- Phototoxicity
- Super-resolution microscopy
- Video microscopy
- Cytoskeleton