MEG3 long noncoding RNA regulates the TGF-β pathway genes through formation of RNA–DNA triplex structures

Mondal, Tanmoy; Subhash, Santhilal; Vaid, Roshan; Enroth, Stefan; Uday, Sireesha; Reinius, Björn; Mitra, Sanhita; Mohammed, Arif; James, Alva Rani; Hoberg, Emily; Moustakas, Aristidis; Gyllensten, Ulf; Jones, Steven J.M.; Gustafsson, Claes M.; Sims, Andrew H.; Westerlund, Fredrik; Gorab, Eduardo; Kanduri, Chandrasekhar

doi:10.1038/ncomms8743

articleNature CommunicationsJul 24, 2015GOLD OA

MEG3 long noncoding RNA regulates the TGF-β pathway genes through formation of RNA–DNA triplex structures

TMTanmoy Mondal SSSanthilal Subhash RVRoshan Vaid SEStefan Enroth SUSireesha Uday

University of Gothenburg · Uppsala University · +6 more institutions

PubMed

Indexed incrossrefdoajpubmed

Abstract

Long noncoding RNAs (lncRNAs) regulate gene expression by association with chromatin, but how they target chromatin remains poorly understood. We have used chromatin RNA immunoprecipitation-coupled high-throughput sequencing to identify 276 lncRNAs enriched in repressive chromatin from breast cancer cells. Using one of the chromatin-interacting lncRNAs, MEG3, we explore the mechanisms by which lncRNAs target chromatin. Here we show that MEG3 and EZH2 share common target genes, including the TGF-β pathway genes. Genome-wide mapping of MEG3 binding sites reveals that MEG3 modulates the activity of TGF-β genes by binding to distal regulatory elements. MEG3 binding sites have GA-rich sequences, which guide MEG3 to…

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Topics & keywords

Topics

Keywords

RNA
Long non-coding RNA
DNA
Non-coding RNA
Gene
Genetics
Biology
MEG3

UN Sustainable Development Goals

Good health and well-being

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