An improvement of the 2ˆ(-delta delta CT) method for quantitative real-time polymerase chain reaction data analysis.
The University of Texas Health Science Center at Houston · The University of Texas MD Anderson Cancer Center
Indexed inpubmed
Abstract
Background
method is applied to data with automatic removal of background fluorescence by the qPCR software. Since the background fluorescence is unknown, subtracting an inaccurate background can lead to distortion of the results. To address these problems, we present an improved method, the individual efficiency corrected calculation.
Results
method.
Conclusions
method and is thus a better way to calculate relative gene expression.
Citation impact
1,599
total citations
- FWCI
- 2.39
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- 100%
- References
- 30
Citations per year
Authors
4Topics & keywords
Topics
Keywords
- Background subtraction
- Real-time polymerase chain reaction
- Subtraction
- Polymerase chain reaction
- Biological system
- Sample (material)
- Computer science
- Mathematics
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