Identification and characterization of essential genes in the human genome
Broad Institute · Allen Institute · +6 more institutions
Abstract
Large-scale genetic analysis of lethal phenotypes has elucidated the molecular underpinnings of many biological processes. Using the bacterial clustered regularly interspaced short palindromic repeats (CRISPR) system, we constructed a genome-wide single-guide RNA library to screen for genes required for proliferation and survival in a human cancer cell line. Our screen revealed the set of cell-essential genes, which was validated with an orthogonal gene-trap-based screen and comparison with yeast gene knockouts. This set is enriched for genes that encode components of fundamental pathways, are expressed at high levels, and contain few inactivating polymorphisms in the human population. We also uncovered a…
Citation impact
- FWCI
- 81.61
- Percentile
- 100%
- References
- 51
Authors
8- TCTimothy C. Wang
Broad Institute, Allen Institute, Whitehead Institute for Biomedical Research, Koch Institute for Integrative Cancer Research At MIT, Massachusetts Institute of Technology
- KBKıvanç Birsoy
Broad Institute, Allen Institute, Whitehead Institute for Biomedical Research, Koch Institute for Integrative Cancer Research At MIT, Massachusetts Institute of Technology
- NWNicholas W. Hughes
Broad Institute
- KMKevin M. Krupczak
Broad Institute, Allen Institute, Whitehead Institute for Biomedical Research, Koch Institute for Integrative Cancer Research At MIT
- YPYorick Post
Broad Institute, Allen Institute, Whitehead Institute for Biomedical Research, Koch Institute for Integrative Cancer Research At MIT
Topics & keywords
- Gene
- Biology
- Genome
- Human genome
- Genetics
- Computational biology
- Robustness (evolution)
- Identification (biology)
- Good health and well-being