articleMolecular Ecology ResourcesJun 20, 2011GREEN OA

DNA barcoding of oomycetes with cytochrome c oxidase subunit I and internal transcribed spacer

Agriculture and Agri-Food Canada · Carleton University · +6 more institutions

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Abstract

Oomycete species occupy many different environments and many ecological niches. The genera Phytophthora and Pythium for example, contain many plant pathogens which cause enormous damage to a wide range of plant species. Proper identification to the species level is a critical first step in any investigation of oomycetes, whether it is research driven or compelled by the need for rapid and accurate diagnostics during a pathogen outbreak. The use of DNA for oomycete species identification is well established, but DNA barcoding with cytochrome c oxidase subunit I (COI) is a relatively new approach that has yet to be assessed over a significant sample of oomycete genera. In this study we have sequenced COI, from…

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