Mechanism of Toxicity in Rotenone Models of Parkinson's Disease
Scripps Research Institute · Emory University · +1 more institution
Abstract
Exposure of rats to the pesticide and complex I inhibitor rotenone reproduces features of Parkinson's disease, including selective nigrostriatal dopaminergic degeneration and alpha-synuclein-positive cytoplasmic inclusions (Betarbet et al., 2000; Sherer et al., 2003). Here, we examined mechanisms of rotenone toxicity using three model systems. In SK-N-MC human neuroblastoma cells, rotenone (10 nm to 1 microm) caused dose-dependent ATP depletion, oxidative damage, and death. To determine the molecular site of action of rotenone, cells were transfected with the rotenone-insensitive single-subunit NADH dehydrogenase of Saccharomyces cerevisiae (NDI1), which incorporates into the mammalian ETC and acts as a…
Citation impact
- FWCI
- 20.90
- Percentile
- 100%
- References
- 52
Authors
10- TSTodd ShererCorresponding
Scripps Research Institute, Emory University, Chosun University
- RBRanjita Betarbet
Scripps Research Institute, Emory University, Chosun University
- CTClaudia Testa
Scripps Research Institute, Emory University, Chosun University
- BBByoung Boo Seo
Scripps Research Institute, Emory University, Chosun University
- JRJason R. Richardson
Scripps Research Institute, Emory University, Chosun University
Topics & keywords
- Rotenone
- Biology
- Dopaminergic
- Oxidative stress
- Parkinson's disease
- Neurodegeneration
- Programmed cell death
- Toxicity
- Good health and well-being