The Transgenic RNAi Project at Harvard Medical School: Resources and Validation

To facilitate large-scale functional studies in Drosophila, the Drosophila Transgenic RNAi Project (TRiP) at Harvard Medical School (HMS) was established along with several goals: developing efficient vectors for RNAi that work in all tissues, generating a genome-scale collection of RNAi stocks with input from the community, distributing the lines as they are generated through existing stock centers, validating as many lines as possible using RT-qPCR and phenotypic analyses, and developing tools and web resources for identifying RNAi lines and retrieving existing information on their quality. With these goals in mind, here we describe in detail the various tools we developed and the status of the collection,…

• HH
  Howard Hughes Medical Institute
• YU
  Yale University
• CS
  Cold Spring Harbor Laboratory
• YU
  York University
• NN
  National Natural Science Foundation of China

  Award: 31371496
• MO
  Ministry of Science and Technology of the People's Republic of China

  Awards: 2015BAI09B03, 2013CB35102, 973 Program
• NI
  National Institutes of Health

  Awards: R01GM067761, P40OD018537, R01GM084947, R37GM062534
• SO
  School of Medicine, New York University
• NK
  National Key Research and Development Program of China

  Award: 2013CB35102
• NO
  NIH Office of the Director

  Award: P40OD018537
• IO
  Institute of Genetics