articleACS NanoDec 18, 2009Closed access

Interaction of Gold Nanoparticles with Common Human Blood Proteins

Center for Biologics Evaluation and Research · United States Food and Drug Administration · +3 more institutions

PubMed
Indexed incrossrefpubmed

Abstract

In order to better understand the physical basis of the biological activity of nanoparticles (NPs) in nanomedicine applications and under conditions of environmental exposure, we performed an array of photophysical measurements to quantify the interaction of model gold NPs having a wide range of NP diameters with common blood proteins. In particular, absorbance, fluorescence quenching, circular dichroism, dynamic light scattering, and electron microscopy measurements were performed on surface-functionalized water-soluble gold NPs having a diameter range from 5 to 100 nm in the presence of common human blood proteins: albumin, fibrinogen, gamma-globulin, histone, and insulin. We find that the gold NPs strongly…

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