ColonyArea: An ImageJ Plugin to Automatically Quantify Colony Formation in Clonogenic Assays
University of Turku · Åbo Akademi University · +1 more institution
Abstract
The clonogenic or colony formation assay is a widely used method to study the number and size of cancer cell colonies that remain after irradiation or cytotoxic agent administration and serves as a measure for the anti-proliferative effect of these treatments. Alternatively, this assay is used to quantitate the transforming potential of cancer associated genes and chemical agents. Therefore, there is a need for a simplified and standardized analysis of colony formation assays for both routine laboratory use and for parallelized automated analysis. Here we describe the freely available ImageJ-plugin "ColonyArea", which is optimized for rapid and quantitative analysis of focus formation assays conducted in 6- to…
Citation impact
- FWCI
- 20.39
- Percentile
- 100%
- References
- 25
Authors
5- CGCamilo Guzmán
University of Turku, Åbo Akademi University, Turku Centre for Biotechnology
- MBManish Bagga
University of Turku, Åbo Akademi University, Turku Centre for Biotechnology
- AKAmanpreet Kaur
Åbo Akademi University, Turku Centre for Biotechnology, University of Turku
- JWJukka Westermarck
University of Turku, Åbo Akademi University, Turku Centre for Biotechnology
- DADaniel AbankwaCorresponding
Turku Centre for Biotechnology, University of Turku, Åbo Akademi University
Topics & keywords
- Clonogenic assay
- Cell counting
- Biology
- Plug-in
- Chemistry
- Computational biology
- Cell
- Computer science