articleProceedings of the National Academy of SciencesDec 10, 2004GREEN OA

A vesicle bioreactor as a step toward an artificial cell assembly

VNVincent NoireauxALAlbert Libchaber

Rockefeller University

PubMed
Indexed incrossrefpubmed

Abstract

An Escherichia coli cell-free expression system is encapsulated in a phospholipid vesicle to build a cell-like bioreactor. Large unilamellar vesicles containing extracts are produced in an oil-extract emulsion. To form a bilayer the vesicles are transferred into a feeding solution that contains ribonucleotides and amino acids. Transcription-translation of plasmid genes is isolated in the vesicles. Whereas in bulk solution expression of enhanced GFP stops after 2 h, inside the vesicle permeability of the membrane to the feeding solution prolongs the expression for up to 5 h. To solve the energy and material limitations and increase the capacity of the reactor, the alpha-hemolysin pore protein from…

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Topics & keywords

Topics
Keywords
  • Vesicle
  • Escherichia coli
  • Bioreactor
  • Vesicle fusion
  • Bilayer
  • Biochemistry
  • Biophysics
  • Chemistry
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