Where Water Is Oxidized to Dioxygen: Structure of the Photosynthetic Mn 4 Ca Cluster
Lawrence Berkeley National Laboratory · Stanford Synchrotron Radiation Lightsource · +3 more institutions
Abstract
The oxidation of water to dioxygen is catalyzed within photosystem II (PSII) by a Mn(4)Ca cluster, the structure of which remains elusive. Polarized extended x-ray absorption fine structure (EXAFS) measurements on PSII single crystals constrain the Mn(4)Ca cluster geometry to a set of three similar high-resolution structures. Combining polarized EXAFS and x-ray diffraction data, the cluster was placed within PSII, taking into account the overall trend of the electron density of the metal site and the putative ligands. The structure of the cluster from the present study is unlike either the 3.0 or 3.5 angstrom-resolution x-ray structures or other previously proposed models.
Citation impact
- FWCI
- 26.44
- Percentile
- 100%
- References
- 29
Authors
11- JYJunko YanoCorresponding
Lawrence Berkeley National Laboratory, Stanford Synchrotron Radiation Lightsource, Technische Universität Berlin, Freie Universität Berlin, University of California, Berkeley
- JKJan KernCorresponding
Lawrence Berkeley National Laboratory, Stanford Synchrotron Radiation Lightsource, Technische Universität Berlin, Freie Universität Berlin, University of California, Berkeley
- KSKenneth Sauer
Lawrence Berkeley National Laboratory, Stanford Synchrotron Radiation Lightsource, Technische Universität Berlin, Freie Universität Berlin, University of California, Berkeley
- MJMatthew J. Latimer
Lawrence Berkeley National Laboratory, Stanford Synchrotron Radiation Lightsource, Technische Universität Berlin, Freie Universität Berlin, University of California, Berkeley
- YPYulia Pushkar
Lawrence Berkeley National Laboratory, Stanford Synchrotron Radiation Lightsource, Technische Universität Berlin, Freie Universität Berlin, University of California, Berkeley
Topics & keywords
- Extended X-ray absorption fine structure
- Oxygen-evolving complex
- Photosystem II
- Cluster (spacecraft)
- Chemistry
- Crystallography
- Manganese
- Absorption (acoustics)
- Clean water and sanitation