Addition of p -Azido- l -phenylalanine to the Genetic Code of Escherichia c oli
Scripps Research Institute · Howard Hughes Medical Institute
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Abstract
We report the selection of a new orthogonal aminoacyl tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in response to the amber codon, TAG. The amino acid is incorporated in good yield with high fidelity and can be used to crosslink interacting proteins.
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Authors
6- JWJason W. ChinCorresponding
Scripps Research Institute, Howard Hughes Medical Institute
- SWStephen W. Santoro
Howard Hughes Medical Institute, Scripps Research Institute
- ABAndrew B. Martin
Scripps Research Institute, Howard Hughes Medical Institute
- DSDavid S. King
Scripps Research Institute, Howard Hughes Medical Institute
- LWLei Wang
Scripps Research Institute, Howard Hughes Medical Institute
Topics & keywords
Topics
Keywords
- Chemistry
- Phenylalanine
- Genetic code
- Transfer RNA
- Escherichia coli
- Amino acid
- Yield (engineering)
- Fidelity
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