Genome-Wide RNAi Analysis of Growth and Viability in Drosophila Cells
Howard Hughes Medical Institute · Harvard University · +2 more institutions
Abstract
A crucial aim upon completion of whole genome sequences is the functional analysis of all predicted genes. We have applied a high-throughput RNA-interference (RNAi) screen of 19,470 double-stranded (ds) RNAs in cultured cells to characterize the function of nearly all (91%) predicted Drosophila genes in cell growth and viability. We found 438 dsRNAs that identified essential genes, among which 80% lacked mutant alleles. A quantitative assay of cell number was applied to identify genes of known and uncharacterized functions. In particular, we demonstrate a role for the homolog of a mammalian acute myeloid leukemia gene (AML1) in cell survival. Such a systematic screen for cell phenotypes, such as cell…
Citation impact
- FWCI
- 27.87
- Percentile
- 100%
- References
- 21
Authors
9- MBMichael BoutrosCorresponding
Howard Hughes Medical Institute, Harvard University, Heidelberg University, Max Planck Institute for Molecular Genetics
- AAAmy A. KigerCorresponding
Howard Hughes Medical Institute, Harvard University, Heidelberg University, Max Planck Institute for Molecular Genetics
- SASusan Armknecht
Howard Hughes Medical Institute, Harvard University, Heidelberg University, Max Planck Institute for Molecular Genetics
- KMKim M. Kerr
Howard Hughes Medical Institute, Harvard University, Heidelberg University, Max Planck Institute for Molecular Genetics
- MHMarc Hild
Howard Hughes Medical Institute, Harvard University, Heidelberg University, Max Planck Institute for Molecular Genetics
Topics & keywords
- RNA interference
- Biology
- Gene
- Genome
- Genetics
- Phenotype
- Viability assay
- Mutant