Efficient TALEN-mediated gene knockout in livestock
University of Minnesota · Recombinetics (United States) · +3 more institutions
Abstract
Transcription activator-like effector nucleases (TALENs) are programmable nucleases that join FokI endonuclease with the modular DNA-binding domain of TALEs. Although zinc-finger nucleases enable a variety of genome modifications, their application to genetic engineering of livestock has been slowed by technical limitations of embryo-injection, culture of primary cells, and difficulty in producing reliable reagents with a limited budget. In contrast, we found that TALENs could easily be manufactured and that over half (23/36, 64%) demonstrate high activity in primary cells. Cytoplasmic injections of TALEN mRNAs into livestock zygotes were capable of inducing gene KO in up to 75% of embryos analyzed, a portion…
Citation impact
- FWCI
- 35.87
- Percentile
- 100%
- References
- 46
Authors
10Topics & keywords
- Transcription activator-like effector nuclease
- Genome editing
- Biology
- Genetics
- Gene knockout
- Zinc finger nuclease
- Gene targeting
- Effector
- Zero hunger