Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation
Harvard University · Cornell University · +1 more institution
Abstract
Multicolor nonlinear microscopy of living tissue using two- and three-photon-excited intrinsic fluorescence combined with second harmonic generation by supermolecular structures produces images with the resolution and detail of standard histology without the use of exogenous stains. Imaging of intrinsic indicators within tissue, such as nicotinamide adenine dinucleotide, retinol, indoleamines, and collagen provides crucial information for physiology and pathology. The efficient application of multiphoton microscopy to intrinsic imaging requires knowledge of the nonlinear optical properties of specific cell and tissue components. Here we compile and demonstrate applications involving a range of intrinsic…
Citation impact
- FWCI
- 61.19
- Percentile
- 100%
- References
- 40
Authors
6- WRWarren R. ZipfelCorresponding
Harvard University, Cornell University, Massachusetts General Hospital
- RMRebecca M. Williams
Harvard University, Cornell University, Massachusetts General Hospital
- RHRichard H. Christie
Harvard University, Cornell University, Massachusetts General Hospital
- AYAlexander Yu. Nikitin
Harvard University, Cornell University, Massachusetts General Hospital
- BTBradley T. Hyman
Harvard University, Cornell University, Massachusetts General Hospital
Topics & keywords
- Microscopy
- Nicotinamide adenine dinucleotide
- Fluorescence
- Two-photon excitation microscopy
- Fluorescence microscope
- Second-harmonic generation
- Fluorescence-lifetime imaging microscopy
- Excited state