articleJournal of the American Chemical SocietyAug 1, 2006GREEN OA

Carbon Nanotube Amplification Strategies for Highly Sensitive Immunodetection of Cancer Biomarkers

XYXin YuBMBernard MungeVPVyomesh PatelGCGary C. JensenABAshwin Bhirde

University of Connecticut · UConn Health

PubMed
Indexed incrossrefpubmed

Abstract

We describe herein the combination of electrochemical immunosensors using single-wall carbon nanotube (SWNT) forest platforms with multi-label secondary antibody-nanotube bioconjugates for highly sensitive detection of a cancer biomarker in serum and tissue lysates. Greatly amplified sensitivity was attained by using bioconjugates featuring horseradish peroxidase (HRP) labels and secondary antibodies (Ab(2)) linked to carbon nanotubes (CNT) at high HRP/Ab(2) ratio. This approach provided a detection limit of 4 pg mL(-)(1) (100 amol mL(-)(1)), for prostate specific antigen (PSA) in 10 microL of undiluted calf serum, a mass detection limit of 40 fg. Accurate detection of PSA in human serum samples was…

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Topics & keywords

Topics
Keywords
  • Detection limit
  • Chemistry
  • Horseradish peroxidase
  • Carbon nanotube
  • Prostate cancer
  • Biomarker
  • Antibody
  • Chromatography
UN Sustainable Development Goals
  • Life in Land
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