MicroRNA Expression in Zebrafish Embryonic Development
University of Copenhagen · McGovern Institute for Brain Research · +4 more institutions
Abstract
MicroRNAs (miRNAs) are small noncoding RNAs, about 21 nucleotides in length, that can regulate gene expression by base-pairing to partially complementary mRNAs. Regulation by miRNAs can play essential roles in embryonic development. We determined the temporal and spatial expression patterns of 115 conserved vertebrate miRNAs in zebrafish embryos by microarrays and by in situ hybridizations, using locked-nucleic acid-modified oligonucleotide probes. Most miRNAs were expressed in a highly tissue-specific manner during segmentation and later stages, but not early in development, which suggests that their role is not in tissue fate establishment but in differentiation or maintenance of tissue identity.
Citation impact
- FWCI
- 38.98
- Percentile
- 100%
- References
- 8
Authors
9- EWErno Wienholds
University of Copenhagen, McGovern Institute for Brain Research, University of Cambridge, The Gurdon Institute, Hubrecht Institute for Developmental Biology and Stem Cell Research, Wellcome Trust
- WPWigard P. Kloosterman
University of Copenhagen, McGovern Institute for Brain Research, University of Cambridge, The Gurdon Institute, Hubrecht Institute for Developmental Biology and Stem Cell Research, Wellcome Trust
- EAEric A. Miska
University of Copenhagen, McGovern Institute for Brain Research, University of Cambridge, The Gurdon Institute, Hubrecht Institute for Developmental Biology and Stem Cell Research, Wellcome Trust
- EAEzequiel Alvarez-Saavedra
University of Copenhagen, McGovern Institute for Brain Research, University of Cambridge, The Gurdon Institute, Hubrecht Institute for Developmental Biology and Stem Cell Research, Wellcome Trust
- EBEugène Berezikov
University of Copenhagen, McGovern Institute for Brain Research, University of Cambridge, The Gurdon Institute, Hubrecht Institute for Developmental Biology and Stem Cell Research, Wellcome Trust
Topics & keywords
- Zebrafish
- Biology
- microRNA
- In situ hybridization
- Gene expression
- Regulation of gene expression
- Vertebrate
- DNA microarray