Bacterial diversity in the oral cavity of 10 healthy individuals
Stanford University · University of California, San Francisco · +7 more institutions
Abstract
The composition of the oral microbiota from 10 individuals with healthy oral tissues was determined using culture-independent techniques. From each individual, 26 specimens, each from different oral sites at a single point in time, were collected and pooled. An 11th pool was constructed using portions of the subgingival specimens from all 10 individuals. The 16S ribosomal RNA gene was amplified using broad-range bacterial primers, and clone libraries from the individual and subgingival pools were constructed. From a total of 11,368 high-quality, nonchimeric, near full-length sequences, 247 species-level phylotypes (using a 99% sequence identity threshold) and 9 bacterial phyla were identified. At least 15…
Citation impact
- FWCI
- 25.75
- Percentile
- 100%
- References
- 43
Authors
10- EMElisabeth M. BikCorresponding
Stanford University
- CDClara Davis Long
Stanford University
- GCGary C. Armitage
University of California, San Francisco
- PMPeter M. Loomer
University of California, San Francisco
- JEJoanne Emerson
J. Craig Venter Institute, Planetary Science Institute, University of California, Berkeley
Topics & keywords
- Biology
- Phylotype
- Phylum
- 16S ribosomal RNA
- Oral cavity
- Ribosomal RNA
- Zoology
- Bacterial phyla
- Life in Land