Heavy metal toxicity: cadmium permeates through calcium channels and disturbs the plant water status

Because plant wilting has been described as a consequence of cadmium (Cd2+) toxicity, we investigate Cd2+ effects on plant water losses, gas exchanges and stomatal behaviour in Arabidopsis thaliana L. Effects of 1-week Cd2+ application in hydroponic condition (CdCl2 10-100 micro m) were analyzed. A 10- micro m Cd2+ concentration had no significant effect on the plant-water relationship and carbon assimilation. At higher Cd2+ concentrations, a Cd2+ -dependent decrease in leaf conductance and CO2 uptake was observed despite the photosynthetic apparatus appeared not to be affected as probed by fluorescence measurements. In epidermal strip bioassays, nanomolar Cd2+ concentrations reduced stomatal opening under…