A Metagenomic Approach to Characterization of the Vaginal Microbiome Signature in Pregnancy
Baylor College of Medicine · Texas Children's Hospital · +3 more institutions
Abstract
While current major national research efforts (i.e., the NIH Human Microbiome Project) will enable comprehensive metagenomic characterization of the adult human microbiota, how and when these diverse microbial communities take up residence in the host and during reproductive life are unexplored at a population level. Because microbial abundance and diversity might differ in pregnancy, we sought to generate comparative metagenomic signatures across gestational age strata. DNA was isolated from the vagina (introitus, posterior fornix, midvagina) and the V5V3 region of bacterial 16S rRNA genes were sequenced (454FLX Titanium platform). Sixty-eight samples from 24 healthy gravidae (18 to 40 confirmed weeks) were…
Citation impact
- FWCI
- 15.58
- Percentile
- 100%
- References
- 63
Authors
14- KMKjersti M. AagaardCorresponding
Baylor College of Medicine, Texas Children's Hospital
- KRKevin Riehle
Texas Children's Hospital, Baylor College of Medicine
- JMJun Ma
Baylor College of Medicine, Texas Children's Hospital
- NSNicola Segata
Harvard University
- TMToni-Ann Mistretta
Baylor College of Medicine, Texas Children's Hospital
Topics & keywords
- Metagenomics
- Biology
- Microbiome
- Clostridiales
- Population
- Phylogenetic diversity
- Lactobacillus crispatus
- Phylogenetic tree