articleProceedings of the National Academy of SciencesOct 14, 2008GREEN OA

MicroRNA-directed transcriptional gene silencing in mammalian cells

DHDaniel H. KimPSPål SætromOSOla SnøveJJJohn J. Rossi

City Of Hope National Medical Center · City of Hope · +2 more institutions

PubMed
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Abstract

MicroRNAs (miRNAs) regulate gene expression at the posttranscriptional level in the cytoplasm, but recent findings suggest additional roles for miRNAs in the nucleus. To address whether miRNAs might transcriptionally silence gene expression, we searched for miRNA target sites proximal to known gene transcription start sites in the human genome. One conserved miRNA, miR-320, is encoded within the promoter region of the cell cycle gene POLR3D in the antisense orientation. We provide evidence of a cis-regulatory role for miR-320 in transcriptional silencing of POLR3D expression. miR-320 directs the association of RNA interference (RNAi) protein Argonaute-1 (AGO1), Polycomb group (PcG) component EZH2, and…

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698
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100%
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Authors

4

Topics & keywords

Topics
Keywords
  • Biology
  • Argonaute
  • Gene silencing
  • RNA-induced silencing complex
  • RNA interference
  • microRNA
  • Regulation of gene expression
  • Epigenetics
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