Highly Variable Chloroplast Markers for Evaluating Plant Phylogeny at Low Taxonomic Levels and for DNA Barcoding
Northeast Forestry University · Chinese Academy of Sciences · +2 more institutions
Abstract
BACKGROUND: At present, plant molecular systematics and DNA barcoding techniques rely heavily on the use of chloroplast gene sequences. Because of the relatively low evolutionary rates of chloroplast genes, there are very few choices suitable for molecular studies on angiosperms at low taxonomic levels, and for DNA barcoding of species. METHODOLOGY/PRINCIPAL FINDINGS: We scanned the entire chloroplast genomes of 12 genera to search for highly variable regions. The sequence data of 9 genera were from GenBank and 3 genera were of our own. We identified nearly 5% of the most variable loci from all variable loci in the chloroplast genomes of each genus, and then selected 23 loci that were present in at least three…
Citation impact
- FWCI
- 7.52
- Percentile
- 100%
- References
- 49
Authors
5- WDWenpan Dong
Northeast Forestry University, Chinese Academy of Sciences, Institute of Botany
- JLJing Liu
Chinese Academy of Sciences, Institute of Botany, University of Chinese Academy of Sciences
- JYJing Yu
University of Chinese Academy of Sciences, Chinese Academy of Sciences, Institute of Botany
- LWLing Wang
Northeast Forestry University
- SZShiliang ZhouCorresponding
Institute of Botany, Chinese Academy of Sciences
Topics & keywords
- Biology
- Intergenic region
- Chloroplast DNA
- NdhF
- DNA barcoding
- GenBank
- Evolutionary biology
- Phylogenetic tree
- Life in Land