Electrospray mass spectrometry of phospholipids

Abstract I. Introduction 332 II. Techniques and Methods 334 A. Sample Preparation 334 B. ESI‐MS/MS 336 1. Glycerophosphocholine (GPCho) Lipids 336 2. Sphingomyelin 341 3. Glycerophosphoethanolamine (GPEtn) Lipids 342 4. Glycerophosphatidic Acid (GPA) Lipids 344 5. Glycerophosphoinositol (GPI) Lipids 346 6. Glycerophosphoglycerol (GPGro) Lipids 349 7. Glycerophosphoserine (GPSer) Lipids 349 8. Cardiolipin 351 C. Matrix‐Assisted Laser Desorption/Ionization (MALDI) Analysis 353 D. Precursor‐Ion and Neutral‐Loss Scanning 353 E. Quantitation 355 III. Research Applications 356 IV. Conclusion 359 Acknowledgments 360 Abbreviations 360 References 360 Phospholipids play a central role in the biochemistry of all living…