Targeting Cre Recombinase to Specific Neuron Populations with Bacterial Artificial Chromosome Constructs

Gong, Shiaoching; Doughty, Martin L.; Harbaugh, Carroll R.; Cummins, Alexander; Hatten, Mary E.; Heintz, Nathaniel; Gerfen, Charles R.

doi:10.1523/jneurosci.2707-07.2007

articleJournal of NeuroscienceSep 12, 2007BRONZE OA

Targeting Cre Recombinase to Specific Neuron Populations with Bacterial Artificial Chromosome Constructs

SGShiaoching Gong MLMartin L. Doughty CRCarroll R. Harbaugh ACAlexander Cummins MEMary E. Hatten

Howard Hughes Medical Institute · National Institute of Mental Health · +1 more institution

PubMed

Indexed incrossrefpubmed

Abstract

Transgenic mouse lines are characterized with Cre recombinase driven by promoters of CNS-specific genes using bacterial artificial chromosome (BAC) constructs. BAC-Cre constructs for 10 genes ( Chat , Th , Slc6a4 , Slc6a2 , Etv1 , Ntsr1 , Drd2 , Drd1 , Pcp2 , and Cmtm5 ) produced 14 lines with Cre expression in specific neuronal and glial populations in the brain. These Cre driver lines add functional utility to the >500 BAC-EGFP (enhanced green fluorescent protein) transgenic mouse lines that are part of the Gene Expression Nervous System Atlas Project.

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Topics & keywords

Topics

Keywords

Bacterial artificial chromosome
Cre recombinase
Recombinase
Biology
Transgene
Green fluorescent protein
Gene
Genetically modified mouse

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