Very Fast Two-Dimensional NMR Spectroscopy for Real-Time Investigation of Dynamic Events in Proteins on the Time Scale of Seconds
Université Joseph Fourier · Commissariat à l'Énergie Atomique et aux Énergies Alternatives · +4 more institutions
Abstract
We demonstrate for different protein samples that 2D 1H-15N correlation NMR spectra can be recorded in a few seconds of acquisition time using a new band-selective optimized flip-angle short-transient heteronuclear multiple quantum coherence experiment. This has enabled us to measure fast hydrogen-deuterium exchange rate constants along the backbone of a small globular protein fragment by real-time 2D NMR.
Citation impact
- FWCI
- 23.32
- Percentile
- 100%
- References
- 15
Authors
2- PSPaul SchandaCorresponding
Université Joseph Fourier, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, Université Grenoble Alpes, Centre National de la Recherche Scientifique, Institut de Biologie Structurale, CEA Grenoble
- BBBernhard Brutscher
Institut de Biologie Structurale, Université Grenoble Alpes, CEA Grenoble, Centre National de la Recherche Scientifique, Université Joseph Fourier, Commissariat à l'Énergie Atomique et aux Énergies Alternatives
Topics & keywords
- Chemistry
- Heteronuclear molecule
- Deuterium NMR
- Two-dimensional nuclear magnetic resonance spectroscopy
- Nuclear magnetic resonance spectroscopy
- Coherence (philosophical gambling strategy)
- Heteronuclear single quantum coherence spectroscopy
- Deuterium