letterJNCI Journal of the National Cancer InstituteMay 3, 2005BRONZE OA

Epidermal Growth Factor Receptor Gene and Protein and Gefitinib Sensitivity in Non–Small-Cell Lung Cancer

Ospedale Bellaria · University of Colorado Denver · +4 more institutions

PubMed
Indexed incrossrefpubmed

Abstract

Background

Gefitinib is a selective inhibitor of the epidermal growth factor (EGFR) tyrosine kinase, which is overexpressed in many cancers, including non-small-cell lung cancer (NSCLC). We carried out a clinical study to compare the relationship between EGFR gene copy number, EGFR protein expression, EGFR mutations, and Akt activation status as predictive markers for gefitinib therapy in advanced NSCLC.

Methods

Tumors from 102 NSCLC patients treated daily with 250 mg of gefitinib were evaluated for EGFR status by fluorescence in situ hybridization (FISH), DNA sequencing, and immunohistochemistry and for Akt activation status (phospho-Akt [P-Akt]) by immunohistochemistry. Time to progression, overall survival, and 95% confidence intervals (CIs) were calculated and evaluated by the Kaplan-Meier method; groups were compared using the log-rank test. Risk factors associated with survival were evaluated using Cox proportional hazards regression modeling and multivariable analysis. All statistical tests were two-sided.

Citation impact

1,569
total citations
FWCI
147.38
Percentile
100%
References
47
Citations per year

Authors

20

Topics & keywords

Keywords
  • Gefitinib
  • Epidermal growth factor receptor
  • Lung cancer
  • Cancer research
  • Gene
  • Epidermal growth factor
  • Biology
  • Cancer
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