The One Hour Yeast Proteome
University of Wisconsin–Madison · Energy Center of Wisconsin · +1 more institution
Abstract
We describe the comprehensive analysis of the yeast proteome in just over one hour of optimized analysis. We achieve this expedited proteome characterization with improved sample preparation, chromatographic separations, and by using a new Orbitrap hybrid mass spectrometer equipped with a mass filter, a collision cell, a high-field Orbitrap analyzer, and, finally, a dual cell linear ion trap analyzer (Q-OT-qIT, Orbitrap Fusion). This system offers high MS(2) acquisition speed of 20 Hz and detects up to 19 peptide sequences within a single second of operation. Over a 1.3 h chromatographic method, the Q-OT-qIT hybrid collected an average of 13,447 MS(1) and 80,460 MS(2) scans (per run) to produce 43,400 (x)…
Citation impact
- FWCI
- 34.85
- Percentile
- 100%
- References
- 49
Authors
7- ASAlexander S. Hebert
University of Wisconsin–Madison, Energy Center of Wisconsin, Molecular Research Institute
- ARAlicia Richards
University of Wisconsin–Madison, Energy Center of Wisconsin
- DJDerek J. Bailey
University of Wisconsin–Madison, Energy Center of Wisconsin
- AUArne Ulbrich
University of Wisconsin–Madison, Energy Center of Wisconsin
- EEEmma E. Coughlin
University of Wisconsin–Madison, Energy Center of Wisconsin
Topics & keywords
- Proteome
- Yeast
- Computational biology
- Chemistry
- Biology
- Biochemistry