Polymorphic Secreted Kinases Are Key Virulence Factors in Toxoplasmosis
Washington University in St. Louis · University of Cambridge · +1 more institution
Abstract
The majority of known Toxoplasma gondii isolates from Europe and North America belong to three clonal lines that differ dramatically in their virulence, depending on the host. To identify the responsible genes, we mapped virulence in F(1) progeny derived from crosses between type II and type III strains, which we introduced into mice. Five virulence (VIR) loci were thus identified, and for two of these, genetic complementation showed that a predicted protein kinase (ROP18 and ROP16, respectively) is the key molecule. Both are hypervariable rhoptry proteins that are secreted into the host cell upon invasion. These results suggest that secreted kinases unique to the Apicomplexa are crucial in the host-pathogen…
Citation impact
- FWCI
- 16.77
- Percentile
- 100%
- References
- 25
Authors
8- JPJeroen P. J. SaeijCorresponding
Washington University in St. Louis, University of Cambridge, Stanford University
- JPJ. P. BoyleCorresponding
Washington University in St. Louis, University of Cambridge, Stanford University
- SCSusan Coller
Washington University in St. Louis, University of Cambridge, Stanford University
- STS. Taylor
Washington University in St. Louis, University of Cambridge, Stanford University
- LDL. David Sibley
Washington University in St. Louis, University of Cambridge, Stanford University
Topics & keywords
- Virulence
- Biology
- Toxoplasma gondii
- Rhoptry
- Complementation
- Gene
- Kinase
- Hypervariable region
- Life below water