Endogenous RNAs Modulate MicroRNA Sorting to Exosomes and Transfer to Acceptor Cells
École Polytechnique Fédérale de Lausanne · SIB Swiss Institute of Bioinformatics · +1 more institution
Abstract
MicroRNA (miRNA) transfer via exosomes may mediate cell-to-cell communication. Interestingly, specific miRNAs are enriched in exosomes in a cell-type-dependent fashion. However, the mechanisms whereby miRNAs are sorted to exosomes and the significance of miRNA transfer to acceptor cells are unclear. We used macrophages and endothelial cells (ECs) as a model of heterotypic cell communication in order to investigate both processes. RNA profiling of macrophages and their exosomes shows that miRNA sorting to exosomes is modulated by cell-activation-dependent changes of miRNA target levels in the producer cells. Genetically perturbing the expression of individual miRNAs or their targeted transcripts promotes…
Citation impact
- FWCI
- 20.56
- Percentile
- 100%
- References
- 49
Authors
8Topics & keywords
- Microvesicles
- microRNA
- Cell biology
- Dicer
- Exosome
- Biology
- Cell
- Cell type