articleNucleic Acids ResearchMay 23, 2010GOLD OA

GUIDANCE: a web server for assessing alignment confidence scores

OPO.C.K.M. PennEPEran PrivmanHAHaim AshkenazyGLGiddy LandanDGDan Graur

Tel Aviv University · University of Houston

PubMed
Indexed incrossrefdoajpubmed

Abstract

Evaluating the accuracy of multiple sequence alignment (MSA) is critical for virtually every comparative sequence analysis that uses an MSA as input. Here we present the GUIDANCE web-server, a user-friendly, open access tool for the identification of unreliable alignment regions. The web-server accepts as input a set of unaligned sequences. The server aligns the sequences and provides a simple graphic visualization of the confidence score of each column, residue and sequence of an alignment, using a color-coding scheme. The method is generic and the user is allowed to choose the alignment algorithm (ClustalW, MAFFT and PRANK are supported) as well as any type of molecular sequences (nucleotide, protein or…

Citation impact

628
total citations
FWCI
10.98
Percentile
100%
References
26
Citations per year

Authors

6

Topics & keywords

Topics
Keywords
  • Web server
  • Multiple sequence alignment
  • Sequence alignment
  • Robustness (evolution)
  • Computer science
  • Biology
  • Visualization
  • Confidence interval
No related works found for this paper.

Funding