articleGenes & DevelopmentFeb 1, 2006DIAMOND OA

Binding of a novel SMG-1–Upf1–eRF1–eRF3 complex (SURF) to the exon junction complex triggers Upf1 phosphorylation and nonsense-mediated mRNA decay

IKIsao KashimaAYAkio YamashitaNINatsuko IzumiNKNaoyuki KataokaRMRyo Morishita

Howard Hughes Medical Institute · Kyoto University · +4 more institutions

PubMed
Indexed incrossrefpubmed

Abstract

Nonsense-mediated mRNA decay (NMD) is a surveillance mechanism that degrades mRNA containing premature termination codons (PTCs). In mammalian cells, recognition of PTCs requires translation and depends on the presence on the mRNA with the splicing-dependent exon junction complex (EJC). While it is known that a key event in the triggering of NMD is phosphorylation of the trans-acting factor, Upf1, by SMG-1, the relationship between Upf1 phosphorylation and PTC recognition remains undetermined. Here we show that SMG-1 binds to the mRNA-associated components of the EJC, Upf2, Upf3b, eIF4A3, Magoh, and Y14. Further, we describe a novel complex that contains the NMD factors SMG-1 and Upf1, and the translation…

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640
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Authors

9

Topics & keywords

Topics
Keywords
  • Nonsense-mediated decay
  • Phosphorylation
  • Biology
  • Cell biology
  • RNA splicing
  • Messenger RNA
  • Poly(A)-binding protein
  • Exon
UN Sustainable Development Goals
  • Life below water
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