articleProceedings of the National Academy of SciencesDec 26, 2002GREEN OA

Mitotic clonal expansion: A synchronous process required for adipogenesis

QTQi-Qun TangTCTamara C. OttoMDM. Daniel Lane

Johns Hopkins University · Johns Hopkins Medicine

PubMed
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Abstract

When induced to differentiate, growth-arrested 3T3-L1 preadipocytes synchronously reenter the cell cycle and undergo mitotic clonal expansion (MCE) followed by expression of genes that produce the adipocyte phenotype. The preadipocytes traverse the G(1)S checkpoint synchronously as evidenced by the expressionactivation of cdk2-cyclin-EA, turnover of p27kip1, hyperphosphorylation of Rb, translocation of cyclin D(1) from nuclei to cytoplasm and GSK-3beta from cytoplasm to nuclei, and incorporation of [(3)H]thymidine into DNA. As the cells cross the G(1)S checkpoint, CEBPbeta acquires DNA-binding activity, initiating a cascade of transcriptional activation that culminates in the expression of adipocyte proteins.…

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Topics & keywords

Topics
Keywords
  • Adipogenesis
  • Cell biology
  • Biology
  • Cyclin
  • Cell cycle
  • Cyclin B1
  • Cyclin A
  • Mitosis
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