Precise and reliable gene expression via standard transcription and translation initiation elements
University of Minho · University of California, Berkeley · +3 more institutions
Abstract
An inability to reliably predict quantitative behaviors for novel combinations of genetic elements limits the rational engineering of biological systems. We developed an expression cassette architecture for genetic elements controlling transcription and translation initiation in Escherichia coli: transcription elements encode a common mRNA start, and translation elements use an overlapping genetic motif found in many natural systems. We engineered libraries of constitutive and repressor-regulated promoters along with translation initiation elements following these definitions. We measured activity distributions for each library and selected elements that collectively resulted in expression across a 1,000-fold…
Citation impact
- FWCI
- 33.50
- Percentile
- 100%
- References
- 85
Authors
11Topics & keywords
- Biology
- Computational biology
- Gene
- Eukaryotic translation
- Genetics
- Promoter
- Transcription (linguistics)
- Gene expression
Funding
- NSNational Science FoundationAwards: 0946510, DE-AC02-05CH11231
- UDU.S. Department of EnergyAwards: -AC02-05CH11231, 05CH11231, AC02-05CH11231, DE-AC02, DE-AC02-05CH11231, DE-AC02-
- FBFondation Bettencourt Schueller
- OOOffice of ScienceAwards: AC02-05CH11231, -AC02-05CH11231, DE-AC02
- FPFundação para a Ciência e a TecnologiaAward: DE-AC02-05CH11231
- BABiological and Environmental ResearchAwards: 05CH11231, DE-AC02-05CH11231, AC02-05CH11231