A crucial role for reactive oxygen species in RANKL-induced osteoclast differentiation
Chonnam National University · Seoul National University · +1 more institution
Abstract
Signaling by receptor activator of NF-kappaB (nuclear factor-kappaB) ligand (RANKL) is essential for differentiation of bone marrow monocyte-macrophage lineage (BMM) cells into osteoclasts. Here, we show RANKL stimulation of BMM cells transiently increased the intracellular level of reactive oxygen species (ROS) through a signaling cascade involving TNF (tumor necrosis factor) receptor-associated factor (TRAF) 6, Rac1, and NADPH (nicotinamide adenine dinucleotide phosphate) oxidase (Nox) 1. A deficiency in TRAF6 or expression of a dominant-interfering mutant of TRAF6 blocks RANKL-mediated ROS production. Application of N-acetylcysteine (NAC) or blocking the activity of Nox, a protein leading to the formation…
Citation impact
- FWCI
- 4.67
- Percentile
- 100%
- References
- 41
Authors
8- NKNa Kyung LeeCorresponding
Chonnam National University, Seoul National University, Chonnam National University Hospital
- YGYoung Geum Choi
Chonnam National University, Seoul National University, Chonnam National University Hospital
- JYJi Youn Baik
Chonnam National University, Seoul National University, Chonnam National University Hospital
- SHSong-Yi Han
Chonnam National University, Seoul National University, Chonnam National University Hospital
- DJDae-won Jeong
Chonnam National University, Seoul National University, Chonnam National University Hospital
Topics & keywords
- RANKL
- Osteoclast
- Cell biology
- Nicotinamide adenine dinucleotide phosphate
- Chemistry
- MAPK/ERK pathway
- RANK Ligand
- Reactive oxygen species