Frequent mutation of histone-modifying genes in non-Hodgkin lymphoma

Follicular lymphoma (FL) and diffuse large B-cell lymphoma (DLBCL) are the two most common non-Hodgkin lymphomas (NHLs). Here we sequenced tumour and matched normal DNA from 13 DLBCL cases and one FL case to identify genes with mutations in B-cell NHL. We analysed RNA-seq data from these and another 113 NHLs to identify genes with candidate mutations, and then re-sequenced tumour and matched normal DNA from these cases to confirm 109 genes with multiple somatic mutations. Genes with roles in histone modification were frequent targets of somatic mutation. For example, 32% of DLBCL and 89% of FL cases had somatic mutations in MLL2, which encodes a histone methyltransferase, and 11.4% and 13.4% of DLBCL and FL…

• UD
  U.S. Department of Health and Human Services

  Award: HHSN261200800001E
• LA
  Leukemia and Lymphoma Society
• GC
  Genome Canada
• CM
  Canada's Michael Smith Genome Sciences Centre
• MS
  Michael Smith Health Research BC
• GB
  Genome British Columbia
• TF
  Terry Fox Foundation

  Award: 019001
• PH
  Provincial Health Services Authority
• NI
  National Institutes of Health

  Awards: Contract No. HHSN261200800001E, HHSN261200800001E
• CI
  Canadian Institutes of Health Research

  Award: TGT-53912
• NC
  National Cancer Institute

  Awards: No. HHSN261200800001E, Contract No. HHSN261200800001E