Peptidoglycan Molecular Requirements Allowing Detection by Nod1 and Nod2

Nod1 and Nod2 are mammalian proteins implicated in the intracellular detection of pathogen-associated molecular patterns. Recently, naturally occurring peptidoglycan (PG) fragments were identified as the microbial motifs sensed by Nod1 and Nod2. Whereas Nod2 detects GlcNAc-MurNAc dipeptide (GM-Di), Nod1 senses a unique diaminopimelate-containing GlcNAc-MurNAc tripeptide muropeptide (GM-TriDAP) found mostly in Gram-negative bacterial PGs. Because Nod1 and Nod2 detect similar yet distinct muropeptides, we further analyzed the molecular sensing specificity of Nod1 and Nod2 toward PG fragments. Using a wide array of natural or modified muramyl peptides, we show here that Nod1 and Nod2 have evolved divergent…

• D
  Danone
• CD
  Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
• MD
  Ministerio de Economía y Competitividad
• CN
  Centre National de la Recherche Scientifique

  Award: UMR 8619