Methylation of tRNA Asp by the DNA Methyltransferase Homolog Dnmt2
University of Southern California · North Carolina State University · +4 more institutions
Abstract
The sequence and the structure of DNA methyltransferase-2 (Dnmt2) bear close affinities to authentic DNA cytosine methyltransferases. A combined genetic and biochemical approach revealed that human DNMT2 did not methylate DNA but instead methylated a small RNA; mass spectrometry showed that this RNA is aspartic acid transfer RNA (tRNA(Asp)) and that DNMT2 specifically methylated cytosine 38 in the anticodon loop. The function of DNMT2 is highly conserved, and human DNMT2 protein restored methylation in vitro to tRNA(Asp) from Dnmt2-deficient strains of mouse, Arabidopsis thaliana, and Drosophila melanogaster in a manner that was dependent on preexisting patterns of modified nucleosides. Indirect sequence…
Citation impact
- FWCI
- 15.27
- Percentile
- 100%
- References
- 22
Authors
9- MGMary GollCorresponding
University of Southern California, North Carolina State University, University of Southern Denmark, Columbia University, Texas A&M University
- FKFinn Kirpekar
University of Southern California, North Carolina State University, University of Southern Denmark, Columbia University, Texas A&M University
- KAKeith A. Maggert
University of Southern California, North Carolina State University, University of Southern Denmark, Columbia University, Texas A&M University
- JAJeffrey A. Yoder
University of Southern California, North Carolina State University, University of Southern Denmark, Columbia University, Texas A&M University
- CHChih-Lin Hsieh
University of Southern California, North Carolina State University, University of Southern Denmark, Columbia University, Texas A&M University
Topics & keywords
- Transfer RNA
- Methyltransferase
- DNA methylation
- Methylation
- Biology
- RNA
- DNA
- RNA-Directed DNA Methylation