articleBiomacromoleculesMar 20, 2015GREEN OA

Protein Corona of Nanoparticles: Distinct Proteins Regulate the Cellular Uptake

Max Planck Society · Max Planck Institute for Polymer Research · +2 more institutions

PubMed
Indexed incrossrefpubmed

Abstract

Understanding nanoparticle-protein interactions is a crucial issue in the development of targeted nanomaterial delivery. Besides unraveling the composition of the nanoparticle's protein coronas, distinct proteins thereof could control nanoparticle uptake into specific cell types. Here we differentially analyzed the protein corona composition on four polymeric differently functionalized nanoparticles by label-free quantitative mass spectrometry. Next, we correlated the relative abundance of identified proteins in the corona with enhanced or decreased cellular uptake of nanoparticles into human cancer and bone marrow stem cells to identify key candidates. Finally, we verified these candidate proteins by…

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