A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
Berkeley College · University of California, Berkeley · +2 more institutions
Abstract
Saccharomyces cerevisiae is an increasingly attractive host for synthetic biology because of its long history in industrial fermentations. However, until recently, most synthetic biology systems have focused on bacteria. While there is a wealth of resources and literature about the biology of yeast, it can be daunting to navigate and extract the tools needed for engineering applications. Here we present a versatile engineering platform for yeast, which contains both a rapid, modular assembly method and a basic set of characterized parts. This platform provides a framework in which to create new designs, as well as data on promoters, terminators, degradation tags, and copy number to inform those designs.…
Citation impact
- FWCI
- 21.23
- Percentile
- 100%
- References
- 54
Authors
4- MEMichael E. LeeCorresponding
Berkeley College, University of California, Berkeley
- WCWilliam C. DeLoache
Berkeley College, University of California, Berkeley
- BCBernardo Cervantes
University of California, Berkeley
- JEJohn E. Dueber
QB3, Lawrence Berkeley National Laboratory, University of California, Berkeley
Topics & keywords
- Synthetic biology
- Modular design
- Yeast
- Computer science
- Saccharomyces cerevisiae
- Set (abstract data type)
- Computational biology
- Metabolic engineering