Methods for Detection of Mitochondrial and Cellular Reactive Oxygen Species

SIGNIFICANCE: Mitochondrial and cellular reactive oxygen species (ROS) play important roles in both physiological and pathological processes. Different ROS, such as superoxide (O2(•-)), hydrogen peroxide, and peroxynitrite (ONOO(-)), stimulate distinct cell-signaling pathways and lead to diverse outcomes depending on their amount and subcellular localization. A variety of methods have been developed for ROS detection; however, many of these methods are not specific, do not allow subcellular localization, and can produce artifacts. In this review, we will critically analyze ROS detection and present advantages and the shortcomings of several available methods. RECENT ADVANCES: In the past decade, a number of…

• NI
  National Institutes of Health

  Awards: HL094469, HL058000, PO-1 HL058000