Genomewide Analysis of PRC1 and PRC2 Occupancy Identifies Two Classes of Bivalent Domains
Broad Institute · Harvard University · +8 more institutions
Abstract
In embryonic stem (ES) cells, bivalent chromatin domains with overlapping repressive (H3 lysine 27 tri-methylation) and activating (H3 lysine 4 tri-methylation) histone modifications mark the promoters of more than 2,000 genes. To gain insight into the structure and function of bivalent domains, we mapped key histone modifications and subunits of Polycomb-repressive complexes 1 and 2 (PRC1 and PRC2) genomewide in human and mouse ES cells by chromatin immunoprecipitation, followed by ultra high-throughput sequencing. We find that bivalent domains can be segregated into two classes -- the first occupied by both PRC2 and PRC1 (PRC1-positive) and the second specifically bound by PRC2 (PRC2-only). PRC1-positive…
Citation impact
- FWCI
- 30.57
- Percentile
- 100%
- References
- 66
Authors
17- MKManching Ku
Broad Institute, Harvard University, Massachusetts General Hospital, Center for Cancer Research
- RPRichard P. Koche
Broad Institute, Massachusetts General Hospital, Harvard–MIT Division of Health Sciences and Technology, Center for Cancer Research
- EREsther Rheinbay
Broad Institute, Boston University, Massachusetts General Hospital, Center for Cancer Research
- EMEric M. Mendenhall
Broad Institute, Harvard University, Massachusetts General Hospital, Center for Cancer Research
- MEMitsuhiro Endoh
RIKEN Center for Integrative Medical Sciences
Topics & keywords
- PRC2
- Biology
- Chromatin
- Polycomb-group proteins
- Epigenetics
- Bivalent (engine)
- DNA methylation
- Genetics